Micellar liquid chromatographic determination of felodipine in tablets and human plasma with fluorescence detection: application to stability studies and content uniformity testing

摘要: A simple and sensitive stability indicating HPLC method was developed validated for the quantitative determination of felodipine (FLP) in presence its degradation products, using atenolol as an internal standard. The separation performed on a C18 column micellar mobile phase consisting 85 mM sodium dodecyl sulfate, 25 phosphate buffer 6.5% pentanol at pH 7. Fluorescence detection set 240 nm (excitation) 440 (emission) used. good linear response achieved range 0.05–15 μg mL−1, with lower limit (LOD) 0.011 mL−1 quantification (LOQ) 0.032 mL−1. suggested successfully applied analysis FLP commercial tablets, mean % recovery value 100.69 ± 0.24%. extended to vitro spiked human plasma samples 99.62 0.51%. utilized investigate kinetics alkaline induced degradation. Moreover, content uniformity testing adopting USP guidelines.