Method and compositions for efficient and specific rolling circle amplification

摘要: Disclosed are compositions and methods for nucleic acid amplification reactions that reduce, prevent, or eliminate artifacts; increase efficiency; specificity; and/or consistency. The disclosed method can combine, example, the use of open circle probes form intramolecular stem structures; matched probe sets in same reaction; detection primers during a plurality rolling replication primer, secondary DNA strand displacement primer common peptide quenchers associated with primers. Such combinations produce, reaction, benefits each combined components.