IL‐10 lentivirus‐laden hydrogel tubes increase spinal progenitor survival and neuronal differentiation after spinal cord injury

摘要: A complex cellular cascade characterizes the pathophysiological response following spinal cord injury (SCI) limiting regeneration. Biomaterial and stem cell combination therapies together have shown synergistic effects, compared to independent benefits of each intervention, represent a promising approach towards regaining function after injury. In this study, we combine our polyethylene glycol (PEG) delivery platform with lentiviral-mediated overexpression anti-inflammatory cytokine interleukin (IL)-10 improve mouse embryonic Day 14 (E14) progenitor transplant survival. Immediately in SCI hemisection model, five PEG tubes were implanted followed by direct injection into lentivirus encoding for IL-10. Two weeks tube implantation, E14 progenitors injected directly integrated tubes, which served as soft substrate transplantation. Together, IL-10 improved survival, assessed at 2 posttransplantation (4 postinjury). On average, 8.1% survived mice receiving lentivirus-laden 0.7% transplants without an 11.5-fold difference. Surviving gave rise neurons when tubes. Axon elongation remyelination observed, addition significant increase functional recovery only control 4 postinjury. All other conditions did not exhibit increased stepping until 8 or 12 This system affords over transplantation microenvironment, offering potential cell-mediated tissue