Prevalence and distribution of Cryptosporidium and Giardia in wastewater and the surface, drinking and ground waters in the Lower Rhine, Germany.

摘要: Samples from different water sources (n = 396) were collected during 2009 and 2011. Wastewater (2-5 l) was purified by aluminium sulphate flocculation. Surface, ground drinking waters (400-6400 filtration. Cryptosporidium oocysts Giardia cysts further concentrated sucrose centrifugation. (Oo)cysts identified IFT (immunofluorescence test), DAPI (4',6-diamidino-2-phenylindole) staining DICM (difference interference contrast microscopy). Out of 206 wastewater samples, 134 (65·0%) found to be positive for 64 (31·1%) oocysts. Parasite numbers ranged 0 2436 cysts/l 1745 oocysts/l. Eight (4·2%) surface samples 190) (0-56000/100 l), 18 (9·5%) (2400/100 l). The purpose this study establish the prevalence concentrations lamblia spp. detecting (oo)cysts samples. This provides substantial evidence that G. are able enter circulate in aquatic environment with negative implications public health.