Determination of pore diameter and molecular weight cut-off of hydrogel-membrane liquid-core capsules for immunoisolation.
作者： Radoslaw Dembczynski , Tomasz Jankowski
DOI: 10.1163/156856201753252552
关键词:
摘要: The exclusion limit expressed as the largest pore size of capsules composed hydrogel Ca-alginate membrane and hydroxy-propyl-ammonium starch liquid core considered immunoprotective system has been determined by means inverse chromatography with dextran molecular weight standards. limits were not influenced change concentration in solution from 4 to 6% but alginate 0.5 1.0%, causing membranes be less permeable. It was found that diameter pores range 7.2-8.0 nm. Based on relationship between solute its weight, had an approximate 21-25 kD for 78-103 protein, which is sufficient block antibodies penetrating through membrane.
sci-hub.se 参考文章(17)
Michael V. Sefton, William T. K. Stevenson, Microencapsulation of live animal cells using polyacrylates Springer, Berlin, Heidelberg. pp. 143- 197 ,(1993) , 10.1007/BFB0027553
Hasan Uludag, Michael V. Sefton, Microencapsulated human hepatoma (HepG2) cells: in vitro growth and protein release. Journal of Biomedical Materials Research. ,vol. 27, pp. 1213- 1224 ,(1993) , 10.1002/JBM.820271002
G. A. King, A. J. Daugulis, P. Faulkner, M. F. A. Goosen, Alginate-Polylysine Microcapsules of Controlled Membrane Molecular Weight Cutoff for Mammalian Cell Culture Engineering Biotechnology Progress. ,vol. 3, pp. 231- 240 ,(1987) , 10.1002/BTPR.5420030407
J. Klein, J. Stock, K. -D. Vorlop, Pore size and properties of spherical Ca-alginate biocatalysts European Journal of Applied Microbiology and Biotechnology. ,vol. 18, pp. 86- 91 ,(1983) , 10.1007/BF00500829
Ronald P. Frigon, John K. Leypoldt, Sharon. Uyeji, Lee W. Henderson, Disparity between Stokes radii of dextrans and proteins as determined by retention volume in gel permeation chromatography Analytical Chemistry. ,vol. 55, pp. 1349- 1354 ,(1983) , 10.1021/AC00259A037
M. Briššová, M. Petro, I. Lacı́k, A.C. Powers, T. Wang, Evaluation of Microcapsule Permeability via Inverse Size Exclusion Chromatography Analytical Biochemistry. ,vol. 242, pp. 104- 111 ,(1996) , 10.1006/ABIO.1996.0435
Wang, New technologies for bioartificial organs. Artificial Organs. ,vol. 22, pp. 68- 74 ,(1998) , 10.1046/J.1525-1594.1998.06056.X
M. Tse, H. Uludag, M. V. Sefton, P. L. Chang, Secretion of recombinant proteins from hydroxyethyl methacrylate‐methyl methacrylate capsules Biotechnology and Bioengineering. ,vol. 51, pp. 271- 280 ,(1996) , 10.1002/(SICI)1097-0290(19960805)51:3<271::AID-BIT3>3.0.CO;2-A
A. Martinsen, G. Skjåk-Braek, O. Smidsrød, Alginate as immobilization material: I. Correlation between chemical and physical properties of alginate gel beads Biotechnology and Bioengineering. ,vol. 33, pp. 79- 89 ,(1989) , 10.1002/BIT.260330111
Taylor Wang, Igor Lacík, Marcela Brissová, Amrutur V. Anilkumar, Ales Prokop, David Hunkeler, Ray Green, Keivan Shahrokhi, Alvin C. Powers, An encapsulation system for the immunoisolation of pancreatic islets Nature Biotechnology. ,vol. 15, pp. 358- 362 ,(1997) , 10.1038/NBT0497-358