Identification of hub genes related to the recovery phase of irradiation injury by microarray and integrated gene network analysis.

摘要: Background Irradiation commonly causes long-term bone marrow injury charactertized by defective HSC self-renewal and a decrease in reserve. However, the effect of high-dose IR on global gene expression during recovery remains unknown. Methodology Microarray analysis was used to identify differentially expressed genes that are likely be critical for recovery. Multiple bioinformatics analyses were conducted key hub genes, pathways biological processes. Principal Findings 1) We identified 1302 murine at 3, 7, 11 21 days after irradiation. Eleven these known associated including Adipoq, Ccl3, Ccnd1, Ccnd2, Cdkn1a, Cxcl12, Junb, Pten, Tal1, Thy1 Tnf; 2) These function multiple processes immunity, hematopoiesis response stimuli, cellular cell proliferation, differentiation, adhesion signaling; 3) Dynamic Gene Network subgroup 25 core participate immune response, regulation transcription nucleosome assembly; 4) A comparison our data with irradiation-related extracted from literature showed 42 matched results microarray analysis, thus demonstrated consistency between studies; 5) Protein-protein interaction network pathway indicated several essential protein-protein interactions signaling pathways, focal immune-related pathways. Conclusions Comparisons other array datasets indicate profiles irradiation damaged show significant differences phases. Our suggest (including hematopoiesis) can considered as process Several members or networks comprehensive analysis.