Dissection of functional domains of adenovirus DNA polymerase by linker-insertion mutagenesis

摘要: Linker-insertion mutations were introduced into the cloned adenovirus DNA polymerase gene and functional effects on initiation elongation of in vitro measured. Essential regions appear to be scattered patches across entire molecule are not limited five homology shared with a variety other replicating polymerases. Thus, presumably contains active sites that must formed by distant interactions molecule.