1,25-Dihydroxyvitamin D3 modulates calcium transport in goat mammary epithelial cells in a dose- and energy-dependent manner

摘要: Calcium is a vital mineral and an indispensable component of milk for ruminants. The regulation transcellular calcium transport by 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3, the active form vitamin D) has been confirmed in humans rodents, regulators, including D receptor (VDR), binding protein D9k (calbindin-D9k), plasma membrane Ca2+-ATPase 1b (PMCA1b), PMAC2b Orai1, are involved this process. However, it still unclear whether 1,25-(OH)2D3 could stimulate ruminant mammary gland. present trials were conducted to study effect supplementation energy availability on expression genes proteins related secretion goat epithelial cells. An vitro culture method secreting cells was successfully established. treated with different doses (0, 0.1, 1.0, 10.0 100.0 nmol/L) research, followed 3-bromopyruvate (3-BrPA, inhibitor glucose metabolism) treatment determine its dependence availability. Cell proliferation ratios, consumption enzyme activities measured commercial kits, real-time quantitative polymerase chain reaction (RT-qPCR), western blots used associated dairy goats, respectively. promoted cell dose-dependent manner when concentration did not exceed 10.0 nmol/L. In addition, 100.0 nmol/L inhibited compared 10.0 nmol/L treatment. inhibition hexokinase 2 (HK2), rate-limiting metabolism, decreased PMCA1b PMCA2b at mRNA levels as well transcription indicating that required transport. optimal facilitated Supplementation influenced regulated modulators dose- energy-dependent manner, thereby highlighting role efficacious regulatory agent produces calcium-enriched ruminants suitable status guaranteed.