Discovery and analysis of inflammatory disease-related genes using cDNA microarrays
作者： R. A. Heller , M. Schena , A. Chai , D. Shalon , T. Bedilion
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摘要: cDNA microarray technology is used to profile complex diseases and discover novel disease-related genes. In inflammatory disease such as rheumatoid arthritis, expression patterns of diverse cell types contribute the pathology. We have monitored gene in this state with a selected human genes probable significance inflammation well expressed peripheral blood cells. Messenger RNA from cultured macrophages, chondrocyte lines, primary chondrocytes, synoviocytes provided profiles for cytokines, chemokines, DNA binding proteins, matrix-degrading metalloproteinases. Comparisons between tissue samples arthritis bowel verified involvement many revealed participation cytokine interleukin 3, chemokine Groα metalloproteinase matrix metallo-elastase both diseases. From library, inhibitor 1, ferritin light chain, manganese superoxide dismutase were identified differentially compared disease. These results successfully demonstrate use system general approach dissecting
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