Anchoring of Vascular Endothelial Growth Factor to Surface-Immobilized Heparin on Pancreatic Islets: Implications for Stimulating Islet Angiogenesis

摘要: In pancreatic islet transplantation, early revascularization is necessary for long-term graft function. We have shown in vitro and vivo models that modification with surface-attached heparin protects the islets from acute attack by innate immune system of blood following intraportal transplantation. this study, we investigated ability an immobilized conjugate composed to bind angiogenic growth factor vascular endothelial factor-A (VEGF-A) as a means attracting cells (ECs) induce angiogenesis revascularization. analyzed capacity VEGF-A how affected proliferation adherence ECs both artificial glass surfaces islets. Quartz crystal microbalance dissipation monitoring slot-blot demonstrated binding heparin-coated upon which showed protein-dependent proliferation. Also, cultured on exhibited effects focal contacts. Heparinized combined unaffected insulin release. Further, covering also increased adhesion surface. Immobilized surface may be useful anchor molecule achieving complete coverage factors, ultimately improving engraftment