ANALYTICAL INFORMATION FROM TITRATION CURVES

摘要: Publisher Summary This chapter provides analytical information from titration curves. ONE of the earliest properties proteins to be subjected physico-chemical measurement was ability combine with acids and bases. Although it is now recognized that this property a combination protein hydrogen ions, terms acid- base-binding capacity are still commonly used. The normal method obtaining curve involved potentiometric both solution solvent acid base, which ions combined or released were calculated. Since time, there have been no striking alterations either in theoretical practical methods determination There has an increased acceptance their use as tool, extension application. displays relation between number protons bound pH environment; while possible measure latter directly, necessary calculate former. It convenient practice select starting point for titrations not far removed neutrality, consider side alkaline hydroxyl ions.