Combination of immunomagnetic separation with real-time PCR for rapid detection of Salmonella in milk, ground beef, and alfalfa sprouts.

摘要: A rapid, specific, and sensitive method for detecting Salmonella spp. in pasteurized milk, ground beef, alfalfa sprouts was developed. The combined immunomagnetic separation with a real-time PCR assay based on the double-stranded DNA binding dye SYBR Green I. primers used produced product melting temperature of 87 ± 0.5°C during by amplifying 284-bp sequence from invasive gene (invA) Salmonella. successful 20 strains, but expected not formed any 11 other bacterial strains. To test this monitoring Salmonella, enterica serotype Newport inoculated into 52 samples each sprouts. Following 10-h nonselective enrichment step buffered peptone water, cells were removed extracted using High Pure template preparation kit. as rea...