Pdsg1 and Pdsg2, Novel Proteins Involved in Developmental Genome Remodelling in Paramecium

摘要: The epigenetic influence of maternal cells on the development their progeny has long been studied in various eukaryotes. Multicellular organisms usually provide zygotes not only with nutrients but also functional elements required for proper development, such as coding and non-coding RNAs. These maternally deposited RNAs exhibit a variety functions, from regulating gene expression to assuring genome integrity. In ciliates, Paramecium these participate programming large-scale reorganization during distinguishing germline-limited DNA, which is excised, somatic-destined DNA. Only handful proteins playing roles this process have identified so far, including typical RNAi-derived factors Dicer-like Piwi proteins. Here we report characterize two novel proteins, Pdsg1 Pdsg2 (Paramecium protein involved Development Somatic Genome 1 2), reorganization. We show that are necessary excision DNA survival sexual progeny. Knockdown PDSG1 PDSG2 genes affects populations small known be elimination (scanRNAs iesRNAs) chromatin modification patterns development. Our results suggest an association between RNA-mediated trans-generational signal modifications