Human Cardiac Mesenchymal Stromal Cells From Right and Left Ventricles Display Differences in Number, Function, and Transcriptomic Profile

摘要: Background Left ventricle (LV) and right (RV) are characterized by well-known physiological differences, mainly related to their different embryological origin, hemodynamic environment, function, structure, cellular composition. Nevertheless, scarce information is available about peculiarities between left ventricular chambers in pathological contexts. Cardiac mesenchymal stromal cells (C-MSC) key affecting many functions of the heart. Differential features that distinguish LV from RV C-MSC still underappreciated. Aim To analyze differential amount, transcriptome human versus (vs.) RV. Methods Human cardiac specimens healthy donors were used for tissue analysis number, isolation. Paired compared as surface marker expression, cell proliferation/death ratio, migration, differentiation capabilities, profile. Results Histological showed a greater percentage vs. tissue. Moreover, higher amount was obtained than after isolation procedures. similar proportion markers. Functional studies revealed comparable growth curves both ventricles. Conversely, displayed apoptosis rate migration speed collagen deposition. Consistently, genes regulation or extracellular matrix organization integrins over-expressed RV, respectively. Besides, we additional pathways specifically associated with C-MSC, including energy metabolism, inflammatory response, conduction, pluripotency. Conclusion Taken together, these results contribute functional characterization conditions. This suggests possible role compartment chamber-specific pathologic scenarios.