Isolation and characterization of UDP-galactose:N-acetylglucosamine 4 beta-galactosyltransferase activity induced in rat parotid glands treated with isoproterenol.
作者： M G Humphreys-Beher
DOI: 10.1016/S0021-9258(18)91084-7
关键词:
摘要: Chronic injection of the beta-adrenergic receptor agonist isoproterenol causes a 6- to 10-fold increase in specific activity Golgi membrane marker enzyme UDP-galactose:D-glucose 4-beta-D-galactosyltransferase (EC 2.4.1.22) rat parotid glands. Using combination size exclusion chromatography and affinity on alpha-lactalbumin-agarose, Triton X-100-solubilized was purified homogeneity from treated control animals. The both sources had single Mr = 60,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. isolated isoproterenol-treated animals identical amino acid compositions isoelectric points (pI 5.9). Neutral sugar content phenol-sulfuric assay 10%. UDP-galactose:N-acetylglucosamine 4 beta-galactosyltransferase alpha-lactalbumin column inhibited presence 200 mM N-acetylglucosamine inclusion assays using 10 acceptor. Increased immune precipitated prepared versus This also true protein synthesized vitro translation poly(A+) RNA glands, indicating an mRNA levels for enzyme. molecular mass immune-precipitated, product be 45,000 Da.
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