Reaction between sheep liver mitochondrial aldehyde dehydrogenase and a chromogenic 'reporter group' reagent.

摘要: The cytosolic form of aldehyde dehydrogenase (ALDH-1) has been shown to react slowly with p-nitrophenyl dimethylcarbamate, liberating p-nitrophenoxide and giving an inactive from the enzyme in which Cys-302 carries a -CO-NMe2 label (Kitson et al., 1991) is work led idea that cyclic analogue carbamate would constitute ‘eporter group’ reagent type originally envisaged by Burr Koshland (1964), since chromophoric moiety end up covalently bound within ene’s active site. compound question, namely 3,4-dihydro-3-methyl-6-ni- tro-2-l,3-benzoxazin-2-one or DMNB (see Figure 1) was synthesised expected way esterases such as chymotrypsin Freeman, 1993). Furtheork ALDH-1 showed this case pKa p-nitrophenol reporter group perturbed upwards about 3 pH units Kitson, 1994). This rather dramatic observation interpreted mean substrate binding site either very hydrophobic character contains negatively charged amino acid sidechain (or conceivably both characteristics).