Cellular and hormonal disruption of fetal testis development in sheep reared on pasture treated with sewage sludge.

摘要: The purpose of this study was to evaluate whether experimental exposure pregnant sheep a mixture environmental chemicals added pasture as sewage sludge (n = 9 treated animals) exerted effects on fetal testis development or function; application undertaken so maximize the ewes its contents. Control 9) were reared with an equivalent amount inorganic nitrogenous fertilizer. Treatment had no effect body weight ewes, but it reduced by 12–15% in male 12) and female 8) fetuses gestation day 110. In 11), significantly (32%), numbers Sertoli cells (34% reduction), Leydig (37% gonocytes (44% compared control 8). Fetal blood levels testosterone inhibin A also (36% 38%, respectively) fetuses, whereas luteinizing hormone follicle-stimulating unchanged. Based immunoexpression anti-Mullerian hormone, cytochrome P450 side chain cleavage enzyme, cell cytoplasmic volume, we conclude that changes probably result from reduction somatic numbers. This could growth restriction and/or lowered action; α-smooth muscle actin peritubular androgen receptor testes animals supports latter possibility. These findings indicate developing fetus real-world mixtures can major attenuation testicular hormonal function, which may have consequences adulthood.