Mechanical Ventilation–induced Diaphragmatic Atrophy Is Associated with Oxidative Injury and Increased Proteolytic Activity

摘要: Prolonged mechanical ventilation (MV) results in reduced diaphragmatic maximal force production and atrophy. To investigate the mechanisms responsible for MV-induced atrophy, we tested hypothesis that controlled MV oxidation of proteins increased proteolysis due to elevated protease activity. Further, postulated would result atrophy all muscle fiber types. Mechanically ventilated animals were anesthetized, tracheostomized, with 21% O2 18 hours. resulted a decrease (p < 0.05) myofibrillar protein cross-sectional area types (i.e., I, IIa, IId/x, IIb). promoted an increase degradation along calpain 20S proteasome Finally, was also associated rise both lipid peroxidation. These data support hypothe...