Nonsense mutation in open reading frame E2 of bovine papillomavirus DNA.

摘要: Oligonucleotide-directed mutagenesis was used to construct a nonsense mutation in open reading frame (ORF) E2 of bovine papillomavirus DNA. A single base substitution constructed which converted TAC codon into TAG amber stop at position the ORF that did not overlap with any other viral ORFs. Full-length DNA containing induced only approximately 2% transformed foci mouse C127 cells were by wild-type In different transformation assay, one-half had acquired mutant gave rise colonies least some morphology. The maintained cell lines derived from DNA, but appeared be integrated host genome. Genetic mapping experiments proved caused decrease focus-forming activity and integration These results suggest encodes protein is involved either directly or indirectly aspects oncogenic maintaining as plasmid cells.