Relocalization of Drosophila dorsal protein can be induced by a rise in cytoplasmic calcium concentration and the expression of constitutively active but not wild-type Toll receptors.

摘要: The generation of dorso-ventral polarity in Drosophila relies on the formation a nuclear gradient rel/nuclear factor kappa B transcription dorsal pre-cellular syncitial embryo by process differential localization. It is thought that formed activation at ventral positions membrane receptor Toll turn causes local dissociation from cytoplasmic anchor protein cactus. Although related its domain to interleukin-1 little known about signal transduction pathways lead relocalization dorsal. In this paper we have used immunofluorescence microscopy as direct assay localization cell line Schneider 2. We find increased calcium concentration and expression constitutively active receptors can induce By contrast, endogenous kinase A wild-type receptors, which activate zen-chloramphenicol acetyltransferase reporter genes system, only marginal effect cellular distribution protein. Treatment cells with activators C radical oxygen intermediates, both B, also has propose different threshold levels be established distinctly regulated pathways.