Effects of niflumic acid on the proliferation of human hepatoma cells
作者： Ling Tao , Yu-Zhen Hu , Ling Dong , Yun-Xin Cao , An-Gang Yang
DOI:
关键词:
摘要: The purpose of this work was to investigate the effects niflumic acid (NFA), a chloride channel blocker, on proliferation human hepatoma cell line (HHCC). Cell analyzed by count and MTT assay. cycle analysis carried out flow cytometry. [Ca(2+)](i) determined laser scanning confocal system. It found that NFA decreased significantly number optical density (OD) HHCC cells, OD value reversed after washout NFA. Compared with control, blocked progression in G(1) phase. Extracellular application (100 micromol/L) induced rapid decrease [Ca(2+)](i). These findings demonstrate blockage channels induces growth arrest phase, which may be due inhibition Ca(2+)/CaM-dependent signaling pathways.
参考文章(7)
M. Mazzanti, L. J. DeFelice, E. F. Smith, Ion channels in murine nuclei during early development and in fully differentiated adult cells The Journal of Membrane Biology. ,vol. 121, pp. 189- 198 ,(1991) , 10.1007/BF01870532
Harmeet Malhi, Adil N. Irani, Pankaj Rajvanshi, Sylvia O. Suadicani, David C. Spray, Thomas V. McDonald, Sanjeev Gupta, KATPChannels Regulate Mitogenically Induced Proliferation in Primary Rat Hepatocytes and Human Liver Cell Lines Journal of Biological Chemistry. ,vol. 275, pp. 26050- 26057 ,(2000) , 10.1074/JBC.M001576200
Shigeomi Shimizu, Yosuke Matsuoka, Yasuo Shinohara, Yoshihiro Yoneda, Yoshihide Tsujimoto, Essential Role of Voltage-Dependent Anion Channel in Various Forms of Apoptosis in Mammalian Cells Journal of Cell Biology. ,vol. 152, pp. 237- 250 ,(2001) , 10.1083/JCB.152.2.237
Robert Wondergem, Wei Gong, Scott H. Monen, Sean N. Dooley, Joel L. Gonce, Tracy D. Conner, Molly Houser, Tom W. Ecay, Kenneth E. Ferslew, Blocking swelling-activated chloride current inhibits mouse liver cell proliferation. The Journal of Physiology. ,vol. 532, pp. 661- 672 ,(2001) , 10.1111/J.1469-7793.2001.0661E.X
Patricia Price, Trevor J. McMillan, Use of the Tetrazolium Assay in Measuring the Response of Human Tumor Cells to Ionizing Radiation Cancer Research. ,vol. 50, pp. 1392- 1396 ,(1990)
Michael D. Ward, William N. Hait, Toni Gestone Parmer, Effects of rottlerin, an inhibitor of calmodulin-dependent protein kinase III, on cellular proliferation, viability, and cell cycle distribution in malignant glioma cells Cell Growth & Differentiation. ,vol. 8, pp. 327- 334 ,(1997)
Joseph R. Hume, Dayue Duan, Mei Lin Collier, Jun Yamazaki, Burton Horowitz, Anion transport in heart Physiological Reviews. ,vol. 80, pp. 31- 81 ,(2000) , 10.1152/PHYSREV.2000.80.1.31