Essential Role of Voltage-Dependent Anion Channel in Various Forms of Apoptosis in Mammalian Cells
作者： Shigeomi Shimizu , Yosuke Matsuoka , Yasuo Shinohara , Yoshihiro Yoneda , Yoshihide Tsujimoto
关键词: Transfection 、 Cytochrome c 、 Mitochondrial apoptosis-induced channel 、 Bcl-2 Homologous Antagonist-Killer Protein 、 Molecular biology 、 Voltage-dependent anion channel 、 Apoptosis 、 Mitochondrion 、 Biology 、 Bcl-2-associated X protein 、 Cell biology
摘要: Through direct interaction with the voltage-dependent anion channel (VDAC), proapoptotic members of Bcl-2 family such as Bax and Bak induce apoptogenic cytochrome c release in isolated mitochondria, whereas BH3-only proteins Bid Bik do not directly target VDAC to release. To investigate biological significance for apoptosis mammalian cells, we produced two kinds anti-VDAC antibodies that inhibited activity. In these prevented Bax-induced loss mitochondrial membrane potential (Δψ), but Bid-induced When microinjected into antibodies, control also apoptosis, did prevent indicating is essential Bax-induced, Bid-induced, changes apoptotic cell death. addition, microinjection significantly etoposide-, paclitaxel-, staurosporine-induced apoptosis. Furthermore, used show Bax- Bak-induced lysis red blood cells was mediated by on plasma membrane. Taken together, our data provide evidence plays an role cells.
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Koko Murakami, Sergio Piomelli, Identification of the cDNA for human red blood cell-specific hexokinase isozyme. Blood. ,vol. 89, pp. 762- 766 ,(1997) , 10.1182/BLOOD.V89.3.762
N. Imamoto, T. Shimamoto, T. Takao, T. Tachibana, S. Kose, M. Matsubae, T. Sekimoto, Y. Shimonishi, Y. Yoneda, In vivo evidence for involvement of a 58 kDa component of nuclear pore-targeting complex in nuclear protein import. The EMBO Journal. ,vol. 14, pp. 3617- 3626 ,(1995) , 10.1002/J.1460-2075.1995.TB00031.X
Mitsuyoshi Saito, Stanley J. Korsmeyer, Paul H. Schlesinger, BAX-dependent transport of cytochrome c reconstituted in pure liposomes. Nature Cell Biology. ,vol. 2, pp. 553- 555 ,(2000) , 10.1038/35019596
Shigeomi Shimizu, Masashi Narita, Yoshihide Tsujimoto, Yoshihide Tsujimoto, Bcl-2 family proteins regulate the release of apoptogenic cytochrome c by the mitochondrial channel VDAC Nature. ,vol. 399, pp. 483- 487 ,(1999) , 10.1038/20959
Atan Gross, Vamsi K. Mootha, Tullia Lindsten, Mona Ashiya, Craig B. Thompson, Michael C. Wei, Stanley J. Korsmeyer, Solly Weiler, tBID, a membrane-targeted death ligand, oligomerizes BAK to release cytochrome c Genes & Development. ,vol. 14, pp. 2060- 2071 ,(2000) , 10.1101/GAD.14.16.2060
Yoshihide Tsujimoto, Hikaru Matsuda, Toshinori Ito, Shigeomi Shimizu, Masaya Nomura, Masashi Narita, Apoptotic cytosol facilitates Bax translocation to mitochondria that involves cytosolic factor regulated by Bcl-2. Cancer Research. ,vol. 59, pp. 5542- 5548 ,(1999)
J. M. Adams, The Bcl-2 Protein Family: Arbiters of Cell Survival Science. ,vol. 281, pp. 1322- 1326 ,(1998) , 10.1126/SCIENCE.281.5381.1322
Paolo Bernardi, Kimberly M. Broekemeier, Douglas R. Pfeiffer, Recent progress on regulation of the mitochondrial permeability transition pore; a cyclosporin-sensitive pore in the inner mitochondrial membrane. Journal of Bioenergetics and Biomembranes. ,vol. 26, pp. 509- 517 ,(1994) , 10.1007/BF00762735
Sharon L. Schendel, Rustam Azimov, Krzysztof Pawłowski, Adam Godzik, Bruce L. Kagan, John C. Reed, ION CHANNEL ACTIVITY OF THE BH3 ONLY BCL-2 FAMILY MEMBER, BID Journal of Biological Chemistry. ,vol. 274, pp. 21932- 21936 ,(1999) , 10.1074/JBC.274.31.21932
Matthew G. Vander Heiden, Navdeep S. Chandel, Edward K. Williamson, Paul T. Schumacker, Craig B. Thompson, Bcl-xL Regulates the Membrane Potential and Volume Homeostasis of Mitochondria Cell. ,vol. 91, pp. 627- 637 ,(1997) , 10.1016/S0092-8674(00)80450-X