Direct voltammetry and catalysis with Mycobacterium tuberculosis catalase-peroxidase, peroxidases, and catalase in lipid films.

摘要: Stable films of dimyristoylphosphatidylcholine and M. tuberculosis catalase−peroxidase (KatG), several peroxidases, myoglobin, catalase showed reversible FeIII/FeII voltammetry on pyrolytic graphite electrodes catalytic current for hydrogen peroxide oxygen. Amperometric responses these to H2O2 at 0 V are likely contain significant contributions from reduction oxygen produced during the cycles. Relative apparent turnover rates pH 6 based steady-state currents versus SCE in presence were order horseradish peroxidase > cytochrome c (CcP) soybean myoglobin KatG catalase. Lower very efficient scavengers may be related instability their compounds I H2O2. catalyzed electrochemical more efficiently than CcP but less other peroxidases. DMPC incorporating glucose oxid...