Size distribution of fullerenol nanoparticles in cell culture medium and their influence on antioxidative enzymes in Chinese hamster ovary cells
作者： Branislava Srdjenovic , Marija Slavic , Karmen Stankov , Nebojsa Kladar , Danica Jovic
关键词:
摘要: Fullerenol (C60(OH)24) nanoparticles (FNP) have a significant role in biomedical research due to their numerous biological activities, some of which cytoprotective and antioxidative properties. The aim this study was measure distribution fullerenol zeta potential cell medium RPMI 1640 with 10% fetal bovine serum (FBS) investigate the influence FNP on Chinese hamster ovary cells (CHO-K1) survival, as well determine activity three enzymes: superoxide-dismutase, glutathione-reductase glutathione-S-transferase mitomycin C-treated line. Our investigation implies that FNP, strong antioxidant, influences cellular redox state enzyme activities thus may reduce proliferation, w hich confirms could be exploited for its use agent.
ache.org.rs 参考文章(28)
Ernest Beutler, Red cell metabolism: A manual of biochemical methods ,(1975)
Hara P. Misra, Irwin Fridovich, The Role of Superoxide Anion in the Autoxidation of Epinephrine and a Simple Assay for Superoxide Dismutase Journal of Biological Chemistry. ,vol. 247, pp. 3170- 3175 ,(1972) , 10.1016/S0021-9258(19)45228-9
Gordana Bogdanović, Vesna Kojić, Aleksandar Đorđević, Jasna Čanadanović-Brunet, Mirjana Vojinović-Miloradov, Vladimir Vit. Baltić, Modulating activity of fullerol C60(OH)22 on doxorubicin-induced cytotoxicity Toxicology in Vitro. ,vol. 18, pp. 629- 637 ,(2004) , 10.1016/J.TIV.2004.02.010
L. Black, M.C. Berenbaum, Factors affecting the dye exclusion test for cell viability Experimental Cell Research. ,vol. 35, pp. 9- 13 ,(1964) , 10.1016/0014-4827(64)90066-7
David Siegel, Howard Beall, Claudia Senekowitsch, Masaji Kasai, Hitoshi Arai, Neil W. Gibson, David Ross, Bioreductive activation of mitomycin C by DT-diaphorase. Biochemistry. ,vol. 31, pp. 7879- 7885 ,(1992) , 10.1021/BI00149A019
David A. Wink, James B. Mitchell, Chemical biology of nitric oxide: insights into regulatory, cytotoxic, and cytoprotective mechanisms of nitric oxide Free Radical Biology and Medicine. ,vol. 25, pp. 434- 456 ,(1998) , 10.1016/S0891-5849(98)00092-6
Bice Fubini, Mara Ghiazza, Ivana Fenoglio, Physico-chemical features of engineered nanoparticles relevant to their toxicity. Nanotoxicology. ,vol. 4, pp. 347- 363 ,(2010) , 10.3109/17435390.2010.509519
A. Djordjević, M. Vojínović-Miloradov, N. Petranović, A. Devečerski, D. Lazar, B. Ribar, Catalytic Preparation and Characterization of C60Br24 Fullerene Science and Technology. ,vol. 6, pp. 689- 694 ,(1998) , 10.1080/10641229809350229
Liang Yan, Zhanjun Gu, Yuliang Zhao, Chemical Mechanisms of the Toxicological Properties of Nanomaterials: Generation of Intracellular Reactive Oxygen Species Chemistry-an Asian Journal. ,vol. 8, pp. 2342- 2353 ,(2013) , 10.1002/ASIA.201300542
Vukosava Milic Torres, Branislava Srdjenovic, Vesna Jacevic, Viktorija Dragojevic Simic, Aleksandar Djordjevic, Ana Luísa Simplício, Fullerenol C60(OH)24 prevents doxorubicin-induced acute cardiotoxicity in rats Pharmacological Reports. ,vol. 62, pp. 707- 718 ,(2010) , 10.1016/S1734-1140(10)70328-5