Lipid metabolism in Chlamydia trachomatis-infected cells: directed trafficking of Golgi-derived sphingolipids to the chlamydial inclusion.

摘要: Abstract Chlamydia trachomatis undergoes its entire life cycle within an uncharacterized intracellular vesicle that does not fuse with lysosomes. We used a fluorescent Golgi-specific probe, (N-[7-(4-nitrobenzo-2-oxa-1,3-diazole)]) aminocaproylsphingosine (C6-NBD-Cer), in conjunction conventional fluorescence or confocal microscopy to identify interactions between the Golgi apparatus and chlamydial inclusion. observed only close physical association inclusion but eventual presence of metabolite this probe associated chlamydiae themselves. Sphingomyelin, endogenously synthesized from C6-NBD-Cer, was specifically transported incorporated into cell wall chlamydiae. Incorporation sphingolipid by inhibited brefeldin A. Chlamydiae therefore occupy distal receives anterograde vesicular traffic normally bound for plasma membrane. Collectively, data suggest may represent unique compartment trans-Golgi network.