Immunocontraceptive activity guided fractionation and characterization of active constituents of neem (Azadirachta indica) seed extracts.

摘要: A novel approach for immunocontraception by intervention of local cell mediated immunity in the reproductive system using single intrauterine application neem oil has been described earlier. The reversible block fertility was reported to last 107-180 days female Wistar rats (Upadhyay et al., 1990. Antifertility effects administration: method contraception. Proceedings Of Royal Society London B 242, 175-180) and 7-11 months monkeys 1994. Long term contraceptive treatment (IUNT) bonnet monkeys: An alternative devices. Contraception 49, 161-167). present study, describes identification characterization biologically active fraction from seeds (Azadirachta indica A. Juss. Family Meliaceae), responsible above activity adult rats. Initial studies with mechanically extracted solvent extracts have revealed that antifertility constituents low intermediate polarity. hexane extract be (Garg Comparison extraction procedures on immunocontraceptive seed extracts. Journal Ethnopharmacology 22, 87-92). Subsequently, sequentially fractionated through various separation techniques tested at each step. Preparative HPLC used isolating individual components quantities, sufficient characterization. analytical developed standardization fraction. identified a mixture six components, which comprises saturated, mono di-unsaturated free fatty acids their methyl esters. Dose response study performed fractions. nature it completely until 5% concentration. There no systemic toxic effect following administration This first time, proposes an seeds, long blocking after high efficacy.