Resolution of Model Holliday Junctions in Vitro

摘要: General genetic recombination consists of a series enzymatic reactions that together lead to the formation recombinant chromosomes. Several models for based on prototype Holliday model have been proposed account various types recombinants produced during meiosis in fungi, best understood form (Holliday 1964; reviewed by Hotchkiss 1974 and Radding 1978). While these differ way which is initiated, they unifying feature an intermediate recombining helices are connected two single-strands cross junction. This two-stranded crossover has particularly interesting structure can migrate along DNA, thereby making heteroduplex DNA symmetrically joined molecules (Fig. 1 A). Since there no net change energy state as base pairs broken reformed when junction moves early investigators branch migration required little was driven random diffusion. However, studies RecA protein E. coli revealed process may be more equivalent unidirectional walk ATP-dependent axial rotation.