Targeted Sequencing Using Affymetrix CustomSeq Arrays

摘要: This unit provides a basic protocol for oligo hybridization-based sequencing technology and resulting data analysis specific to the Affymetrix GeneChip CustomSeq Resequencing Array platform. All steps critical aspects related array design, experimental protocols, management, base-calling algorithms are addressed. is particularly appropriate targeted regions of genome up 300 kilo-bases. The most suitable detecting substitution mutations, unless indel probes added.