Temporal trends in cyanobacteria revealed through DNA and pigment analyses of temperate lake sediment cores

摘要: Reports of cyanobacterial blooms in temperate lakes have been increasing over the last few decades. If are indeed becoming more frequent or intense, this poses a problem for water and ecosystem management as can be toxic to wildlife humans. Here we used paleolimnological approach determine whether cyanobacteria Western Quebec, region with thousands lack historical surface monitoring data. We compared within outside Gatineau Park, protected conservation area since 1938. Sediment cores dating back pre-European settlement were analyzed temporal trends cyanobacteria, order assess effect land use change and/or climate before after Park’s creation. extracted sediment DNA 16S rRNA gene specific based on qPCR assay absolute copy numbers. These results analyses carotenoid pigments zeaxanthin echinenone, along diatoxanthin β-carotene, representative diatoms all algae respectively. Overall, numbers pointed significant increase five past 30 years also creation, when average (past 150 years). In contrast, eubacterial copies glutamine synthesis indicated that total microbial abundance exhibited relatively smaller same time periods. No difference percent quantified by both pigments, was observed between Park. This would suggest other factors, such warming documented region, may driving cyanobacteria. has potential corroborate classical fossil remains provide novel information structure function ecosystems.