Immunological characterization of lymphocytes in synovial fluid from patients with rheumatoid arthritis.

摘要: Lymphocytes separated from synovial fluid in rheumatoid arthritis by means of gradient centrifugation were characterized with regard to B- and T-lymphocyte properties. Membrane-bound Ig detectable immunofluorescence technique was utilized as a marker B-lymphocytes, while the ability bind sheep erythrocytes form rosettes interpreted characteristic. In 9 out 18 patients studied no Ig-positive lymphocytes found fluid, remaining had 1% or less. Appropriate control experiments indicated that absence membrane-bound on not due vivo induced redistribution subsequent endocytosis receptors. The percentages B-lymphocytes peripheral blood (mean 11% range 3–19%) significantly different those 54 normal individuals 10% 3–22%). Rosette-forming 11 studied, mean 29.6% 19.0–60.2%, which are higher than corresponding values blood, 22.7% 0.9–41.1%. latter also 32 normals 14.8% 0.9–34.7%). increased proportions T-lymphocytes contrast very low numbers may indicate an important role for former pathogenesis inflammation.