Reactivation of an inactive human X chromosome: evidence for X inactivation by DNA methylation

作者: T Mohandas , R. Sparkes , L. Shapiro

DOI: 10.1126/SCIENCE.6164095

关键词:

摘要: A mouse-human somatic cell hybrid clone, deficient in hypoxanthine-guanine phosphoribosyltransferase (HPRT) and containing a structurally normal inactive human X chromosome, was isolated. The cells were treated with 5-azacytidine tested for the reactivation expression of X-linked genes. frequency HPRT-positives clones after treatment 1000-fold greater than that observed untreated cells. Fourteen independent HPRT-positive isolated analyzed markers. Isoelectric focusing showed HPRT expressed these is human. One 14 glucose-6-phosphate dehydrogenase another phosphoglycerate kinase. Since results hypomethylation DNA, DNA methylation may be mechanism chromosome inactivation.

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