Natural killer cell stimulatory factor (NKSF) augments natural killer cell and antibody-dependent tumoricidal response against colon carcinoma cell lines

摘要: The therapy of colorectal cancer may be improved by biologic response modifiers that enhance natural killer (NK) cell and antibody-dependent tumoricidal mechanisms. This study examined the effect a recently discovered cytokine purified from supernatant an Ebstein-Barr virus-transformed B-lymphoblastoid line (RPMI-8866), stimulatory factor (NKSF), on NK cellular cytotoxicity (ADCC) human colon adenocarcinoma lines. Human peripheral blood lymphocytes were cultured for 24 hr in presence or absence NKSF (3.6 pM) interleukin-2 (1 nM). analyzed lytic potential toward chromium-51-labeled carcinoma targets SW 1116, 498 LI, WC 1. ADCC was measured incubating 1116 1 with monoclonal antibody CO17-1A, IgG2a reactive gastrointestinal cancer-associated antigen, control mouse IgG prior to testing NKSF-treated PBL effectors 6-hr assay. significantly enhanced cytolysis NK-resistant lymphoma lines, molar basis approximately 300 times more potent than generating cytotoxicity. Furthermore, augmented lymphocyte-mediated against targets, combination CO17-1A had additive lymphocyte tumoricidial capacity. Thus, have role treatment cancer.