A Patch-Based Method for Repetitive and Transient Event Detection in Fluorescence Imaging
作者： Jérôme Boulanger , Alexandre Gidon , Charles Kervran , Jean Salamero
DOI: 10.1371/JOURNAL.PONE.0013190
关键词:
摘要: Automatic detection and characterization of molecular behavior in large data sets obtained by fast imaging advanced light microscopy become key issues to decipher the dynamic architectures their coordination living cell. quantification number sudden transient events observed fluorescence is discussed this paper. We propose a calibrated method based on comparison image patches expected distinguish appearing/vanishing fluorescent spots from other motion behaviors such as lateral movements. analyze performances two statistical control procedures compare proposed approach frame difference using same controls benchmark synthetic sequences. have then selected model related membrane trafficking considered real sequences cells stably expressing an endocytic-recycling trans-membrane protein, Langerin-YFP, for validation. With model, we targeted efficient local concentration arising base provided different modalities, wide field (WF) video maximum intensity projection along axial direction total internal reflection microscopy. Finally, briefly used statistically explore effect several perturbations rate detected pilot biological model.
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