Characterization of metabolic profile of mosapride citrate in rat and identification of two new metabolites: Mosapride N-oxide and morpholine ring-opened mosapride by UPLC–ESI-MS/MS

摘要: Abstract The identification and structure elucidation of metabolites mosapride, a selective gastroprokinetic agent, was investigated in rats. After oral administration, samples rat urine, bile, feces plasma were collected analyzed by UPLC–ESI-MS/MS method. Altogether 18 detected at least 15 reported for the first time. Two new metabolites, mosapride N-oxide urine plasma, morpholine ring-opened feces, identified comparison with reference standards. One known major mammalian metabolite, des-p-fluorobenzyl also identified. molecular structures nine phase I six II elucidated based on characteristics their protonated ions, product ions chromatographic retention times. mainly transformed four main metabolism pathways, dealkylation, N-oxidation, ring cleavage hydroxylation, dealkylation as predominant metabolic pathway, while formed glucuronidation. relatively comprehensive pathway proposed.