Fungal biotransformation of mosapride by Cunninghamella elegans

摘要: Abstract The filamentous fungus, Cunninghamella elegans AS 3.156, was used as a microbial model of mammalian metabolism to transform mosapride, selective 5-HT4-receptor agonist. fungal metabolites mosapride were separated and detected by ultra performance liquid chromatography–tandem mass spectrometric method. After incubation for 120 h, the parent drug metabolized thirteen metabolites, one which known major metabolite, des-p-fluorobenzyl while others all novel metabolites. Four including three new metabolite in mammals isolated using preparative high-performance chromatography identified 1D- 2D-nuclear magnetic resonance, UV spectroscopic analysis. Other characterized according their chromatographic behavior, spectral data. metabolic pathways transformed fungus N-oxidation, morpholinyl ring cleavage, N-dealkylation p-fluorobenzyl further N-formylation, minor N-dealkylation, glucoside conjugation, sulfate formylation opened followed acetylation. fungi belonging species could not only be generate microgram scale, but also facilitate production putative or derivatives. isolates reference standards identification analytical tests mosapride.