Isolation and Characterization of Proteoglycans from Human Nonepithelial Tumors

摘要: Proteoglycans (PGs) and glycosaminoglycans (GAGs) were identified in myogenic fibrogenic tumors. More PGs containing mainly chondroitin sulfate could be detected malignant tumors (leiomyosarcomas) than benign (leiomyomas fibromas). Two groups of the by ion-exchange chromatography gel chromatography. One group was a large molecule with side chains, seemingly composed two or more subpopulations that eluted from Sepharose CL-4B kav 0.45. After removal GAG chains PG chondroitinase AC digestion, core molecules molecular weights greater 200,000 obtained. Another fraction small It consisted weight ≃48,000 sulfate-dermatan hybrids. The mixed sequence l-iduronic acid d-glucuronic same chain demonstrated formation proportion tetrasaccharide after digestion. In tumors, found only very amounts, one Its protein had ≃46,000, which similar to obtained leiomyosarcomas, but its dermatan amounts glucuronic acid. results suggest both are products gene they processed different manner form proteoglycans types chains.