Clinical application of a rapid microbiological test based on capillary zone electrophoresis to assess local skin infection

作者: Jacek Szeliga , Marek Jackowski , Ewa Kłodzińska , Bogusław Buszewski , Wojciech Kupczyk

DOI: 10.1186/1756-0500-4-467

关键词:

摘要: Background: The basic clinical problem associated with infection treatment is the fact that classic, commonly and routinely used isolation identification methods are based on long-term processes of a phenotypic analysis microorganisms. Consequently sometimes, especially in small centres, rapid implementation antibacterial becomes delayed. work presents initial results microbiological an original method capillary zone electrophoresis (CZE). study involved 78 biological samples from post-operative wounds trophic ulcers. Results: attempt was made to identify individual bacterial species characteristic features electropherograms achieved. Finally, G(+) cocci type bacteria different G(-) rods were identified sensitivity 88.1% specificity 100%. Conclusions: Based trials using electrophoretic technique field diagnostics infected exudate wound it can be concluded relatively sensitive for infectious pathogens.

参考文章(11)
Michał Szumski, Ewa Kłodzińska, Bogusław Buszewski, Separation of microorganisms using electromigration techniques. Journal of Chromatography A. ,vol. 1084, pp. 186- 193 ,(2005) , 10.1016/J.CHROMA.2004.09.062
H. Richardson, F. Smaill, Recent advances: medical microbiology. BMJ. ,vol. 317, pp. 1060- 1062 ,(1998) , 10.1136/BMJ.317.7165.1060
Jiru Xu, John E Moore, Philip G Murphy, B Cherie Millar, J Stuart Elborn, Early detection of Pseudomonas aeruginosa – comparison of conventional versus molecular (PCR) detection directly from adult patients with cystic fibrosis (CF) Annals of Clinical Microbiology and Antimicrobials. ,vol. 3, pp. 21- 21 ,(2004) , 10.1186/1476-0711-3-21
Gi Won Shin, Yang Sook Cho, Hee Sung Hwang, Mi-Hwa Oh, Hong Gil Nam, Jin Hyun Park, Gyoo Yeol Jung, A new single-step quantitative pathogen detection system: template-tagging followed by multiplex asymmetric PCR using common primers and CE-SSCP. Electrophoresis. ,vol. 30, pp. 2728- 2736 ,(2009) , 10.1002/ELPS.200900074
Bogusław Buszewski, Michał Szumski, Ewa Kłodzińska, Hanna Dahm, Separation of bacteria by capillary electrophoresis Journal of Separation Science. ,vol. 26, pp. 1045- 1049 ,(2003) , 10.1002/JSSC.200301442
Wai S. Law, Sam F.Y. Li, Larry J. Kricka, Detection of enteropathogenic Escherichia coli by microchip capillary electrophoresis. Methods of Molecular Biology. ,vol. 509, pp. 169- 179 ,(2009) , 10.1007/978-1-59745-372-1_12
Marek Jackowski, Jacek Szeliga, Ewa Kłodzińska, Bogusław Buszewski, Application of capillary zone electrophoresis (CZE) to the determination of pathogenic bacteria for medical diagnosis Analytical and Bioanalytical Chemistry. ,vol. 391, pp. 2153- 2160 ,(2008) , 10.1007/S00216-008-2021-0
Ewa Kłodzińska, Hanna Dahm, Henryk Róźycki, Jacek Szeliga, Marek Jackowski, Bogusław Buszewski, Rapid identification of Escherichia coli and Helicobacter pylori in biological samples by capillary zone electrophoresis Journal of Separation Science. ,vol. 29, pp. 1180- 1187 ,(2006) , 10.1002/JSSC.200500351
Alex Belkum, Nicole H.M. Renders, Saskia Smith, Shelley E. Overbeek, Henri A. Verbrugh, Comparison of conventional and molecular methods for the detection of bacterial pathogens in sputum samples from cystic fibrosis patients. Fems Immunology and Medical Microbiology. ,vol. 27, pp. 51- 57 ,(2000) , 10.1111/J.1574-695X.2000.TB01411.X