A teleostean counterpart of ferritin M subunit from rock bream (Oplegnathus fasciatus): an active constituent in iron chelation and DNA protection against oxidative damage, with a modulated expression upon pathogen stress.

摘要: Ferritins are biological iron chelators that can sequestrate excess to maintain homeostasis in the body. basically consist of 2 types subunits, designated as H and L. However, another new subunit, ferritin "M" which possesses characteristic features both L was recently identified lower vertebrates, mostly fish. In this study, a M-like subunit from rock bream (Oplegnathus fasciatus) (RbFerM) characterized at molecular level, its transcriptional profile analyzed healthy fish, well pathogen- mitogen-stimulated Furthermore, functional properties were evaluated using recombinant protein. The complete coding sequence RbFerM 528 bp length, encoding 176-amino acid peptide with calculated mass 20 kDa. silico analysis revealed it has similar mammalian Phylogenetic depicted higher evolutionary proximity fish counterparts. Quantitative real time polymerase chain reaction (PCR) detected ubiquitous selected tissues bream, more pronounced expression observed blood liver tissues. Significant inductions upon lipopolysaccharides (LPS), Edwardsiella tarda, Streptococcus iniae, irido virus (RBIV) exposures time-course immune-challenge experiments. purified protein demonstrated detectable chelating activity varied temperature. Moreover, rendered protection effect against (II) H2O2-mediated DNA damage.