Analysis of genetic diversity in Indian taro [ Colocasia esculenta (L.) Schott] using random amplified polymorphic DNA (RAPD) markers

作者: S. Lakhanpaul , K.C. Velayudhan , K.V. Bhat

DOI: 10.1023/A:1024498408453

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摘要: Taro [Colocasia esculenta (L.) Schott] germplasm accessions collected from different parts of India were subjected to RAPD (Random Amplified Polymorphic DNA) analysis assess the genetic diversity prevalent and also test basis morphotypic classification. Thirteen random decamer primers out 22 tested used analyse 32 taro belonging 28 morphotypes. Three these thirteen analysed showed 100 per cent polymorphism. Per polymorphism varied 60 among polymorphic primers. High was revealed as similarity coefficient values ranged 0.50 0.98. No two in present study a value one thereby indicating their distinctness presence high Indian germplasm. Dendrogram obtained UPGMA grouped four clusters three placed outliers. Clustering pattern did not show any strict relationship with geographical distribution, morphotype classification genotypic diversity. Further, classified, same group always cluster together. Presence very close genepool wild, weedy cultivated forms extreme levels phenotypic variation is suggested reason for reported. Usefulness DNA markers such characterising assessing hereby demonstrated.

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