Clinical, genetic, and pharmacogenetic applications of the invader assay*

摘要: The Invader technology has been developed for the detection of nucleic acids. It is a signal amplification system able to accurately quantify DNA and RNA targets with high sensitivity. Exquisite specificity achieved by combining hybridization enzyme recognition, which provides ability discriminate mutant from wild-type at ratios greater than 1/1000 (mutant/wt). isothermal flexible incorporates homogeneous fluorescence readout. therefore readily adaptable use in clinical reference laboratories, as well high-through-put applications using 96-, 384-, 1,536-well microtiter plate formats. molecular mechanism specific research laboratories are described. These include direct analysis unamplified human genomic detect mutations single-nucleotide polymorphisms associated factor V Leiden, II, cystic fibrosis, apolipoprotein E, gene expression assays that messenger levels cells lysates.