Effects of the addition of oocyte meiosis-inhibiting drugs on the expression of maturation-promoting factor components and organization of cytoplasmic organelles.

摘要: The present study evaluated the effects of blockade meiosis in bovine oocytes by cyclin-dependent kinase inhibitors roscovitine (ROS) and butyrolactone-I (BL-I) on nuclear maturation extracellular signal-regulated 1/2 (ERK1/2), cyclin B1 p34cdc2 protein expression localization. We also ultrastructural changes oocytes. Immature were obtained from slaughtered bovines divided into: (1) control (oocytes for vitro only tissue culture medium-199 24 h), (2) that treated with 12.5μMROS 6 h, (3) 50μMBL-I h (4) 6.25 μMROS+25 μMBL-I h. Incubation was followed reversal 18 Oocytes then underwent immunohistochemical analysis to visualize chromatin assess ERK1/2, localization/expression, preparation cells ultrastructure electron microscopy. groups at before IVM exhibited lowest number metaphase I. ROS group had highest degenerating (p 0.05). differed throughout inhibition oocyte < No difference observed localization these proteins ooplasm. between treatments, exception treatment drugs augmented lipid metabolism Results indicate CDK1 are reversible oocytes, indicated nuclear, cytoplasmic, molecular parameters.