High throughput and multiplex localization of proteins and cells for in situ micropatterning using pneumatic microfluidics
作者： Jian-Chun Wang , Wenming Liu , Qin Tu , Chao Ma , Lei Zhao
DOI: 10.1039/C4AN01972E
关键词:
摘要: Micropatterning technologies are emerging as an enabling tool for various microfluidic-based applications in life sciences. However, the high throughput and multiplex localization of multiple bio-components a microfluidic device has not yet been well established. In this paper, we describe simple situ micropatterning method using integrated with pneumatic microstructures (PμSs) highly controllable immobilization both proteins cells throughput, geometry-dynamic, multi-patterning way. The precise Pluronic F127 passivation microchamber surface except PμS-blocked regions was performed characterized, spatial dynamics consistency PμSs protein/cell were optically evaluated quantitatively demonstrated too. Furthermore, systematic investigation PμS-assisted microfluidics carried out. feature control can be simply realized by well-designed PμS arrays. Meanwhile, co-micropatterning different (bovine serum albumin chicken egg albumin) (human umbilical vein endothelial human hepatocellular carcinoma cells) successfully accomplished orderly serial manipulation groups. We demonstrate that applied convenient component large-scale diversified patterning manipulation, which could useful cell-based tissue organization, high-throughput imaging, protein-related interactions immunoassays.
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