Transient expression of functional serotonin 5‐HT3 receptors by glutamatergic granule cells in the early postnatal mouse cerebellum

摘要: Non-Technical Summary  The serotonin 3 receptor is a ligand-gated ion channel which activated by the neurotransmitter serotonin. This present in many parts of brain, yet there hardly any information on its function cerebellum. In this study, we use transgenic mice to show that localised cerebellum during first three weeks after birth. We found period, regulates communication between two major types neurons cerebellum, granule cells and Purkinje cells. absence early postnatal development, synaptic transmission those disturbed. Taken together, these findings can help us understand way affects may provide insight into pathophysiological conditions serotonergic system compromised. Abstract  5-HT3 only expressed GABAergic interneurons brain regions, including cortex, amygdala hippocampus. Furthermore, receptors are glutamatergic Cajal–Retzius cerebral cortex. used 5-HT3A/enhanced green fluorescent protein (EGFP) also ubiquitously weeks. Using whole-cell patch clamp recordings, local application either or selective agonist SR57227A results small inward current, demonstrating post- and/or extrasynaptic localisation receptors. Functional were observed presynaptically at parallel fibre–Purkinje cell synapse. Pharmacological block using antagonist tropisetron induced reduction frequency miniature events recorded from Paired-pulse stimulation fibres voltage clamped 1-week-old did not plasticity. presence tropisetron, synapse showed paired-pulse depression. functional development where they modulate