Effects of chrysolaminarin synthase knockdown in the diatom Thalassiosira pseudonana: Implications of reduced carbohydrate storage relative to green algae

摘要: Abstract In all organisms, the flux of carbon through fundamental pathways glycolysis, gluconeogenesis and pyruvate hub is a core process related to growth productivity. unicellular microalgae, complexity intracellular location specific steps these can vary substantially. addition, chemical nature storage carbohydrate be substantially different. The role starch in green algae has been investigated, but thus far, only minimal understanding diatoms as β-1,3-glucan chrysolaminarin achieved. this report, we aimed determine effect specifically reducing ability Thalassiosira pseudonana cells accumulate by knocking down transcript levels synthase gene. We monitored changes triacylglycerol (TAG) during silicon starvation. Transcript-level genes encoding metabolism, cytoplasmic chloroplast glycolysis/gluconeogenesis, were limitation, highlighting processes involved. demonstrate that knockdown lines less have transiently increased TAG level, with detriment growth. results provide insight into diatoms, further discussion highlights differences between stores on