Silencing UDP-glucose pyrophosphorylase gene in Phaeodactylum tricornutum affects carbon allocation.
作者： Bao-Hua Zhu , Hong-Ping Shi , Guan-Pin Yang , Na-Na Lv , Miao Yang
DOI: 10.1016/J.NBT.2015.06.003
关键词:
摘要: The effects of the suppression UDP-glucose pyrophosphorylase (UGPase) on chrysolaminaran biosynthesis and carbon allocation were investigated in Phaeodactylum tricornutum. 69% decrease UGPase activity was accompanied by a 4.89 fold reduction Ugp transcript abundance. Inactivation P. tricornutum led to significant content an increase lipid synthesis. These findings suggest that is rate-limiting enzyme may play important role chrysolaminarin allocation. Our results support theoretical deduction good candidate for improving synthesis diatoms.
sci-hub.se 参考文章(43)
Henrik Johansson, Gene regulation of UDP-glucose synthesis and metabolism in plants Fysiologisk botanik. ,(2003)
Espen Granum, Sverre M. Myklestad, A simple combined method for determination of β-1,3-glucan and cell wall polysaccharides in diatoms Hydrobiologia. ,vol. 477, pp. 155- 161 ,(2002) , 10.1023/A:1021077407766
Mark Olive, Wolfgang Walter Schuch, Ian Georges Bridges, Adp glucose-pyrophosphorylase ,(1989)
Sing-Lai Lim, Wan-Loy Chu, Siew-Moi Phang, Use of Chlorella vulgaris for bioremediation of textile wastewater. Bioresource Technology. ,vol. 101, pp. 7314- 7322 ,(2010) , 10.1016/J.BIORTECH.2010.04.092
Michel. DuBois, K. A. Gilles, J. K. Hamilton, P. A. Rebers, Fred. Smith, Colorimetric Method for Determination of Sugars and Related Substances Analytical Chemistry. ,vol. 28, pp. 350- 356 ,(1956) , 10.1021/AC60111A017
Melinda J. Griffiths, Clive Garcin, Robert P. van Hille, Susan T.L. Harrison, Interference by pigment in the estimation of microalgal biomass concentration by optical density Journal of Microbiological Methods. ,vol. 85, pp. 119- 123 ,(2011) , 10.1016/J.MIMET.2011.02.005
Paul G. Roessler, UDPGLUCOSE PYROPHOSPHORYLASE ACTIVITY IN THE DIATOM CYCLOTELLA CRYPTICA. PATHWAY OF CHRYSOLAMINARIN BIOSYNTHESIS Journal of Phycology. ,vol. 23, pp. 494- 498 ,(1987) , 10.1111/J.1529-8817.1987.TB02536.X
Alex Y. Borovkov, Phillip E. Mcclean, Joseph R. Sowokinos, Susan H. Ruud, Gary A. Secor, Effect of Expression of UDP-Glucose Pyrophosphorylase Ribozyme and Antisense RNAs on the Enzyme Activity and Carbohydrate Composition of Field-Grown Transgenic Potato Plants Journal of Plant Physiology. ,vol. 147, pp. 644- 652 ,(1996) , 10.1016/S0176-1617(11)81473-2
Lioudmila A. Zaslavskaia, J. Casey Lippmeier, Peter G. Kroth, Arthur R. Grossman, Kirk E. Apt, Transformation of the diatom Phaeodactylum tricornutum (Bacillariophyceae) with a variety of selectable marker and reporter genes Journal of Phycology. ,vol. 36, pp. 379- 386 ,(2001) , 10.1046/J.1529-8817.2000.99164.X
Yantao Li, Danxiang Han, Guongrong Hu, David Dauvillee, Milton Sommerfeld, Steven Ball, Qiang Hu, Chlamydomonas starchless mutant defective in ADP-glucose pyrophosphorylase hyper-accumulates triacylglycerol. Metabolic Engineering. ,vol. 12, pp. 387- 391 ,(2010) , 10.1016/J.YMBEN.2010.02.002