Overexpression of endogenous delta-6 fatty acid desaturase gene enhances eicosapentaenoic acid accumulation in Phaeodactylum tricornutum
作者： Bao-Hua Zhu , Chang-Chao Tu , Hong-Ping Shi , Guan-Pin Yang , Ke-Hou Pan
DOI: 10.1016/J.PROCBIO.2017.03.013
关键词:
摘要: Abstract The effect of overexpression endogenous delta-6 fatty acid desaturase gene ( ER 6FAD ) on eicosapentaenoic (EPA) production and total lipid content was investigated in Phaeodactylum tricornutum . All three randomly selected transformants exhibited significant increase (47.66%) their EPA content, which reached up to 38.101 mg/g DW algal strain D63. all the significantly higher (16.40–18.64%) than that wild-type strain. These findings suggested reaction catalyzed by is a limiting step for biosynthesis, an effective method improving eukaryotic microalgae, metabolic pathway microalgae can be genetically modified.
sci-hub.se 参考文章(47)
Bao-Hua Zhu, Hong-Ping Shi, Guan-Pin Yang, Na-Na Lv, Miao Yang, Ke-Hou Pan, Silencing UDP-glucose pyrophosphorylase gene in Phaeodactylum tricornutum affects carbon allocation. New Biotechnology. ,vol. 33, pp. 237- 244 ,(2016) , 10.1016/J.NBT.2015.06.003
Frédéric Domergue, Jens Lerchl, Ulrich Zähringer, Ernst Heinz, Cloning and functional characterization of Phaeodactylum tricornutum front-end desaturases involved in eicosapentaenoic acid biosynthesis. FEBS Journal. ,vol. 269, pp. 4105- 4113 ,(2002) , 10.1046/J.1432-1033.2002.03104.X
Olga V. Sayanova, Frédéric Beaudoin, Louise V. Michaelson, Peter R. Shewry, Johnathan A. Napier, Identification of Primula fatty acid Δ6‐desaturases with n‐3 substrate preferences1 FEBS Letters. ,vol. 542, pp. 100- 104 ,(2003) , 10.1016/S0014-5793(03)00358-2
Nicole Poulsen, Nils Kröger, A new molecular tool for transgenic diatoms FEBS Journal. ,vol. 272, pp. 3413- 3423 ,(2005) , 10.1111/J.1742-4658.2005.04760.X
G Lepage, C C Roy, Improved recovery of fatty acid through direct transesterification without prior extraction or purification. Journal of Lipid Research. ,vol. 25, pp. 1391- 1396 ,(1984) , 10.1016/S0022-2275(20)34457-6
Melinda J. Griffiths, Clive Garcin, Robert P. van Hille, Susan T.L. Harrison, Interference by pigment in the estimation of microalgal biomass concentration by optical density Journal of Microbiological Methods. ,vol. 85, pp. 119- 123 ,(2011) , 10.1016/J.MIMET.2011.02.005
Alimuddin, Goro Yoshizaki, Viswanath Kiron, Shuichi Satoh, Toshio Takeuchi, Enhancement of EPA and DHA biosynthesis by over-expression of masu salmon Δ6-desaturase-like gene in zebrafish Transgenic Research. ,vol. 14, pp. 159- 165 ,(2005) , 10.1007/S11248-004-7435-7
S.Y. Yu, H. Li, M. Tong, L.L. Ouyang, Z.G. Zhou, Identification of a Δ6 fatty acid elongase gene for arachidonic acid biosynthesis localized to the endoplasmic reticulum in the green microalga Myrmecia incisa Reisigl Gene. ,vol. 493, pp. 219- 227 ,(2012) , 10.1016/J.GENE.2011.11.053
Frédéric Domergue, Patricia Spiekermann, Jens Lerchl, Christoph Beckmann, Oliver Kilian, Peter G Kroth, Wilhem Boland, Ulrich Zähringer, Ernst Heinz, New Insight into Phaeodactylum tricornutum Fatty Acid Metabolism. Cloning and Functional Characterization of Plastidial and Microsomal Δ12-Fatty Acid Desaturases Plant Physiology. ,vol. 131, pp. 1648- 1660 ,(2003) , 10.1104/PP.102.018317
Olga Sayanova, Richard Haslam, Irina Guschina, David Lloyd, William W. Christie, John L. Harwood, Johnathan A. Napier, A Bifunctional Δ12,Δ15-Desaturase from Acanthamoeba castellanii Directs the Synthesis of Highly Unusual n-1 Series Unsaturated Fatty Acids Journal of Biological Chemistry. ,vol. 281, pp. 36533- 36541 ,(2006) , 10.1074/JBC.M605158200