High-efficiency production of bisabolene from waste cooking oil by metabolically engineered Yarrowia lipolytica.

摘要: The natural plant product bisabolene serves as a precursor for the production of wide range industrially relevant chemicals. However, low abundance in plants renders its isolation from sources non-economically viable. Therefore, creation microbial cell factories supported by synthetic biology and metabolic engineering strategies presents more competitive environmentally sustainable method industrial bisabolene. In this proof-of-principle study, first time, we engineered oleaginous yeast Yarrowia lipolytica to produce α-bisabolene, β-bisabolene γ-bisabolene through heterologous expression α-bisabolene synthase Abies grandis, gene Zingiber officinale Helianthus annuus respectively. Subsequently, two approaches, including overexpression endogenous mevalonate pathway genes introduction multidrug efflux transporters, were employed order improve production. Furthermore, fermentation conditions optimized maximize Y. strains glucose. Finally, explored potential waste cooking oil. To our knowledge, is report using strategies. These findings provide valuable insights into higher-level utilization converting oil various products.