Applicability of a keratinocyte gene signature to predict skin sensitizing potential.

摘要: There is a need to replace animal tests for the identification of skin sensitizers and currently many alternative assays are being developed that have very promising results. In this study gene signature capable accurate was established in HaCaT human keratinocyte cell line. This evaluated separate using six chemicals either local lymph node (LLNA) false-positive or false-negative addition nine four non-sensitizers. Similar studies do not apply these more difficult classify chemicals, which show true potential predictions an assay. Although has improved prediction accuracy compared LLNA, misclassified compounds were comparable between two assays. Gene profiling also showed sensitizer specific response Nrf2-keap1 Toll-like receptor signaling pathways. After exposure non-sensitizing induce pathways all Nrf2-inducers, while ligands correctly classified. conclusion, we confirm based may provide essential information on properties compounds. Furthermore, chemical selection critical assessment performance vitro