Cadmium concentrations in the testes, sperm, and spermatids of mice subjected to long-term cadmium chloride exposure.

摘要: Background: Exposures to cadmium have been reported reduce male fertility and there are several hypotheses that suggest how reduced may result from incorporation of into sperm chromatin The purpose this study was determine whether mice subjected long-term intraperitoneal exposure incorporated their chromatin. Methods: Male were exposed 0.1 mg/kg body weight in the form CdCl2 via injection once per week for 4, 10, 26, 52 weeks then sacrificed. contents liver, testes, pooled sperm, spermatids dosed control animals determined by atomic absorption spectroscopy. Cadmium zinc individual spermatid heads particle-induced x-ray emission. Results: Atomic spectroscopy revealed although accumulated liver not detected or samples down minimum detectable limits 0.02 μg/g dry weight. Particle-induced emission analyses did show presence any head 15 also demonstrated phosphorus, sulfur, concentrations altered CdCl2. Conclusions: Because at levels above weight, data cast doubt on chromatin. Cytometry 35:30–36, 1999. Published 1999 Wiley-Liss, Inc.