RNA conformational requirements of self-cleavage of hepatitis delta virus RNA.

摘要: Hepatitis delta virus (HDV) RNA subfragments undergo self-cleavage at varying efficiencies. We have developed a procedure of using repeated cycles heat denaturation and renaturation to achieve high efficiency cleavage. This effect can also be achieved by gradual with or formamide. These results suggest that only subpopulation the catalytic molecules assumes active conformation required for self-cleavage. could general use detecting activities.